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Determination of nitrogen isotopes on samples with tens of nmol of N using the combination of an elemental analyzer, a GasBench interface and an isotope ratio mass spectrometer: An evaluation of blank N contributions and blank-correction
Cui, Linlin1,2; Wang, Xu1,2; Feng, Lianjun1
2019-01-15
发表期刊RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN0951-4198
卷号33期号:1页码:74-80
摘要Rationale: The combination of an Elemental Analyzer, a GasBench interface and Isotope Ratio Mass Spectrometry (EA-GasBench-IRMS system) is a promising method for the delta N-15 analysis of samples containing trace amounts of nitrogen (N). Nevertheless, N blanks, which are limiting factors for the accuracy and precision of measured delta N-15 values, have received little study. In this paper, a variety of N blank sources in the EA-GasBench-IRMS system were systematically evaluated in order to take effective measures to reduce the blank interference as much as possible. Methods: N-Isotopic analysis was accomplished using an elemental analyzer coupled to an isotope ratio mass spectrometer via a sample loop and a GasBench interface. The N in the sample was converted into N-2 gas in the EA system, and then transferred and trapped in a sample loop with a deactivated stainless-steel chromatography column packed with 5 A molecular sieve polymer at liquid nitrogen temperature (-196 degrees C). Subsequently, the N-2 gas was released by warming the sample loop up to 100 degrees C and introduced into the isotope ratio mass spectrometer via the GasBench interface. The N blank sources in the EA-GasBench-IRMS system were investigated systematically by looking at seven parts: (a) Helium carrier gas, (b) Autosampler, (c) CO2/water trap, (d) Size of reactor tube, (e) Sample collection time, (f) Oxygen gas, and (g) Capsules. Results: The N blanks are mainly derived from the helium carrier gas, atmospheric N-2 entrained into the system through the autosampler, and the N retained in the CO2/water trap filled with CO2 absorbent and Mg(ClO4)(2), which together can account for a total of similar to 507.3 nmol N. Through purifying the helium gas, modifying the autosampler and using a cryogenic trap, we reduced the N blank considerably, to similar to 10.7 nmol, and obtained a nearly uniform isotopic composition (delta N-15(Blank) = -4.54 +/- 0.36 parts per thousand AIR, n = 32, 1SD) of blank N thus guaranteeing a reliable correction. Conclusions: Measurements on a set of IAEA-N1, IAEA-600 and collagen standards with 40 nmol-200 nmol N produced accurate delta N-15 values with standard deviation of +/- 0.26 parts per thousand, +/- 0.22 parts per thousand and +/- 0.23 parts per thousand (1 sigma) after blank correction, respectively. Our findings offer clues to optimizing the analytical method for trace N isotopic determination and they are also beneficial to improving delta N-15 measurement using conventional EA-IRMS.
DOI10.1002/rcm.8309
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences
关键词[WOS]DELTA-N-15 ; DELTA-C-13 ; ABUNDANCE ; C-13 ; SOIL
语种英语
资助项目National Natural Science Foundation of China[41603013] ; Instrument Function Developing Project of the Chinese Academy of Sciences[IGG201601]
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Function Developing Project of the Chinese Academy of Sciences ; Instrument Function Developing Project of the Chinese Academy of Sciences
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
WOS类目Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
WOS记录号WOS:000453713000009
出版者WILEY
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文献类型期刊论文
条目标识符http://ir.iggcas.ac.cn/handle/132A11/90273
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通讯作者Cui, Linlin
作者单位1.Chinese Acad Sci, Inst Geol & Geophys, Beijing 100029, Peoples R China
2.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Cenozo Geol & Environm, Beijing 100029, Peoples R China
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Cui, Linlin,Wang, Xu,Feng, Lianjun. Determination of nitrogen isotopes on samples with tens of nmol of N using the combination of an elemental analyzer, a GasBench interface and an isotope ratio mass spectrometer: An evaluation of blank N contributions and blank-correction[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2019,33(1):74-80.
APA Cui, Linlin,Wang, Xu,&Feng, Lianjun.(2019).Determination of nitrogen isotopes on samples with tens of nmol of N using the combination of an elemental analyzer, a GasBench interface and an isotope ratio mass spectrometer: An evaluation of blank N contributions and blank-correction.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,33(1),74-80.
MLA Cui, Linlin,et al."Determination of nitrogen isotopes on samples with tens of nmol of N using the combination of an elemental analyzer, a GasBench interface and an isotope ratio mass spectrometer: An evaluation of blank N contributions and blank-correction".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 33.1(2019):74-80.
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