IGGCAS OpenIR  > 兰州油气中心
Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction
Sajjad, Wasim1,2; Zheng, Guodong1; Ma, Xiangxian1; Rafiq, Muhammad3; Irfan, Muhammad4,5; Xu, Wang1,2; Ali, Barkat2,6
2019-03-01
Source PublicationJOURNAL OF BASIC MICROBIOLOGY
ISSN0233-111X
Volume59Issue:3Pages:323-336
AbstractThe exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous iron-oxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope, China. The morphological, biochemical, physiological, and phylogenetic characteristics of isolates were investigated. These isolates were motile, Gram-negative, and curved shape with pleomorphism except isolate WG101 that was a straight rod. The optimum growth pH and temperature for all isolates were 1.5 and 30 degrees C, respectively, and showed extreme acidophilic nature. All the isolates showed obligate chemoautotrophic nature and used ferrous iron and pyrite as an energy source, however, isolates WG102 and WG103 were unable to use sulfur, while isolate WG101 could use elemental sulfur and reduced inorganic sodium thiosulfate as an energy source. The phylogenetic analyses based on 16S rRNA sequences revealed that the isolates WG101, WG102, and WG103 were homologous with Acidithiobacillus ferrooxidans strain AS2 (99%), Leptospirillum ferriphilum strain YSK (98%), and Leptospirillum ferrooxidans strain L15 (98%), respectively. These bacterial isolates showed efficient copper and zinc dissolution from the ore. The metals dissolution rate of At. ferrooxidans strain WG101 was 54.5 +/- 4.33% (copper) and 49.6 +/- 5% (zinc). The metals recovery rate of L. ferriphilum strain WG102 was 45.7 +/- 3.5% (copper) and 40.5 +/- 2.5% (zinc). The recovery rate of copper and zinc was 49.6 +/- 4% and 46.5 +/- 3% respectively in the case of L. ferrooxidans strain WG103. The findings of this study are consistent with the notion that the indigenous bacteria are more efficient in minerals dissolution.
KeywordAcidithiobacillus ferrooxidans Baiyin Copper Mine bioleaching Leptospirillum ferriphilum Leptospirillum ferrooxidans
DOI10.1002/jobm.201800433
Funding OrganizationCAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China
WOS KeywordPRINTED-CIRCUIT BOARDS ; ACIDITHIOBACILLUS-FERROOXIDANS ; LEPTOSPIRILLUM-FERRIPHILUM ; MIXED CULTURE ; FERROUS IRON ; SOLID MEDIUM ; DRAINAGE ; STRAIN ; OXIDATION ; IDENTIFICATION
Language英语
Funding ProjectCAS-TWAS President's Fellowship ; Natural Science Foundation of China[41572352]
Funding OrganizationCAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000461581400010
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iggcas.ac.cn/handle/132A11/90894
Collection兰州油气中心
Corresponding AuthorZheng, Guodong
Affiliation1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Petr Resources, Gansu Prov Key Lab Petr Resources Res, Lanzhou 730000, Gansu, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Bristol, Sch Geog Sci, Bristol Glaciol Ctr, Fac Sci, Bristol, Avon, England
4.Univ Florida, Dept Microbiol & Cell Sci, Genet Inst, Gainesville, FL 32611 USA
5.Univ Florida, Inst Food & Agr Sci, Gainesville, FL 32611 USA
6.Univ Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryosphere Sci, Lanzhou, Gansu, Peoples R China
Recommended Citation
GB/T 7714
Sajjad, Wasim,Zheng, Guodong,Ma, Xiangxian,et al. Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction[J]. JOURNAL OF BASIC MICROBIOLOGY,2019,59(3):323-336.
APA Sajjad, Wasim.,Zheng, Guodong.,Ma, Xiangxian.,Rafiq, Muhammad.,Irfan, Muhammad.,...&Ali, Barkat.(2019).Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction.JOURNAL OF BASIC MICROBIOLOGY,59(3),323-336.
MLA Sajjad, Wasim,et al."Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction".JOURNAL OF BASIC MICROBIOLOGY 59.3(2019):323-336.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sajjad, Wasim]'s Articles
[Zheng, Guodong]'s Articles
[Ma, Xiangxian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sajjad, Wasim]'s Articles
[Zheng, Guodong]'s Articles
[Ma, Xiangxian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sajjad, Wasim]'s Articles
[Zheng, Guodong]'s Articles
[Ma, Xiangxian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.