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Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake
Li, Jinhua1,2,3; Zhang, Heng1,3; Liu, Peiyu1,3; Menguy, Nicolas4; Roberts, Andrew P.5; Chen, Haitao1,3; Wang, Yinzhao1,3; Pan, Yongxin1,3,6
2019-07-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume85Issue:14Pages:11
AbstractMagnetotactic bacteria (MTB) are phylogenetically diverse prokaryotes that are able to biomineralize intracellular, magnetic chains of magnetite or greigite nanocrystals called magnetosomes. Simultaneous characterization of MTB phylogeny and biomineralization is crucial but challenging because most MTB are extremely difficult to culture. We identify a large rod, bean-like MTB (tentatively named WYHR-1) from freshwater sediments of Weiyang Lake, Xi'an, China, using a coupled fluorescence and scanning electron microscopy approach at the single-cell scale. Phylogenetic analysis of 16S rRNA gene sequences indicates that WYHR-1 is a novel genus from the Deltaproteobacteria class. Transmission electron microscope observations reveal that WYHR-1 cells contain tens of magnetite magnetosomes that are organized into a single chain bundle along the cell long axis. Mature WYHR-1 magnetosomes are bullet-shaped, straight, and elongated along the [001] direction, with a large flat end terminated by a {100} face at the base and a conical top. This crystal morphology is distinctively different from bullet-shaped magnetosomes produced by other MTB in the Deltaproteobacteria class and the Nitrospirae phylum. This indicates that WYHR-1 may have a different crystal growth process and mechanism from other species, which results from species-specific magnetosome biomineralization in MTB. IMPORTANCE Magnetotactic bacteria (MTB) represent a model system for understanding biomineralization and are also studied intensively in biogeomagnetic and paleomagnetic research. However, many uncultured MTB strains have not been identified phylogenetically or investigated structurally at the single-cell level, which limits comprehensive understanding of MTB diversity and their role in biomineralization. We have identified a novel MTB strain, WYHR-1, from a freshwater lake using a coupled fluorescence and scanning electron microscopy approach at the single-cell scale. Our analyses further indicate that strain WYHR-1 represents a novel genus from the Deltaproteobacteria class. In contrast to bullet-shaped magnetosomes produced by other MTB in the Deltaproteobacteria class and the Nitrospirae phylum, WYHR-1 magnetosomes are bullet-shaped, straight, and highly elongated along the [001] direction, are terminated by a large {100} face at their base, and have a conical top. Our findings imply that, consistent with phylogenetic diversity of MTB, bullet-shaped magnetosomes have diverse crystal habits and growth patterns.
Keywordbacterial diversity bullet-shaped magnetosomes coupled fluorescence
DOI10.1128/AEM.00731-19
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant
WOS KeywordBACTERIA REVEALS ; MAGNETITE ; DIVERSITY ; BIOMINERALIZATION ; CRYSTALS ; GREIGITE ; PROKARYOTES ; NITROSPIRAE ; SIGNATURE ; EVOLUTION
Language英语
Funding ProjectNational Natural Science Foundation of China[41890843] ; National Natural Science Foundation of China[41522402] ; National Natural Science Foundation of China[41621004] ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology[MGQNLM201704] ; Australian Research Council[DP140104544] ; Region Ile-de-France grant[SESAME 2000 E 1435]
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000476468600022
PublisherAMER SOC MICROBIOLOGY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iggcas.ac.cn/handle/132A11/92928
Collection地球与行星物理院重点实验室
Corresponding AuthorLi, Jinhua
Affiliation1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Shandong, Peoples R China
3.CNRS CAS, Int Associated Lab Evolut & Dev Magnetotact Multi, Beijing, Peoples R China
4.Sorbonne Univ, UPMC, MNHN, IMPMC,CNRS UMR 7590,IRD UMR 206, Paris, France
5.Australian Natl Univ, Res Sch Earth Sci, Canberra, ACT, Australia
6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
First Author AffilicationKey Laboratory of Earth and Planetary Physics, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Earth and Planetary Physics, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Jinhua,Zhang, Heng,Liu, Peiyu,et al. Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(14):11.
APA Li, Jinhua.,Zhang, Heng.,Liu, Peiyu.,Menguy, Nicolas.,Roberts, Andrew P..,...&Pan, Yongxin.(2019).Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(14),11.
MLA Li, Jinhua,et al."Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.14(2019):11.
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