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Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification
Ullah, Saif1; Irfan, Muhammad2; Sajjad, Wasim3; Rana, Qurrat Ul Ain1; Hasan, Fariha1; Khan, Samiullah1; Badshah, Malik1; Shah, Aamer Ali1
2019-10-02
Source PublicationFOOD BIOTECHNOLOGY
ISSN0890-5436
Volume33Issue:4Pages:353-372
AbstractA xylanase-producing bacterium Bacillus pumilus K22 was isolated from hot spring located in Manghopir area, Karachi, Pakistan. The enzyme was purified to homogeneity by acetone precipitation and size exclusion chromatography. The molecular weight was determined to be approximately 24 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified xylanase retained its activity at wide range of temperature (20-70?C) and pH (5.0?10.0) with maximum stability at 50?C and pH 8.0. Xylanase activity was inhibited by Zn2+, Cu2+, and Hg2+ metal ions while it remained stable in the presence of Mn2+, Ca2+ and Mg2+. The activity was also inhibited by chemical reagents such as EDTA, PMSF, and ?-mercaptoethanol. The enzyme was stable in the presence of all organic solvents (20%) except formaldehyde and glycerol, which inhibited its activity. Treatment of tomato pulp with purified xylanase-increased juice yield (30%), clarity (9%), and reducing sugars (69%), indicating an improvement in physico-chemical characteristics of the tomato pulp.
KeywordBacillus pumilus xylanase size exclusion chromatography SDS-PAGE organic solvents
DOI10.1080/08905436.2019.1674157
Funding OrganizationHigher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan
WOS KeywordCELLULASE-FREE XYLANASE ; ENZYMATIC CLARIFICATION ; MICROBIAL XYLANASES ; SAPODILLA JUICE ; PURIFICATION ; STRAIN ; OPTIMIZATION ; ENZYMES ; CLONING
Language英语
Funding ProjectHigher Education Commission of Pakistan
Funding OrganizationHigher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS IDWOS:000494491700005
PublisherTAYLOR & FRANCIS INC
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Document Type期刊论文
Identifierhttp://ir.iggcas.ac.cn/handle/132A11/95735
Collection兰州油气中心
Corresponding AuthorShah, Aamer Ali
Affiliation1.Quaid I Azam Univ, Fac Biol Sci, Dept Microbiol, Islamabad 45320, Pakistan
2.Sarhad Univ Sci & Informat Technol, Inst Biol Sci, Peshawar, Pakistan
3.Chinese Acad Sci, Inst Geol & Geophys, Gansu Prov Key Lab Petr Resources Res, Key Lab Petr Resources, Lanzhou, Gansu, Peoples R China
Recommended Citation
GB/T 7714
Ullah, Saif,Irfan, Muhammad,Sajjad, Wasim,et al. Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification[J]. FOOD BIOTECHNOLOGY,2019,33(4):353-372.
APA Ullah, Saif.,Irfan, Muhammad.,Sajjad, Wasim.,Rana, Qurrat Ul Ain.,Hasan, Fariha.,...&Shah, Aamer Ali.(2019).Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification.FOOD BIOTECHNOLOGY,33(4),353-372.
MLA Ullah, Saif,et al."Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification".FOOD BIOTECHNOLOGY 33.4(2019):353-372.
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