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Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1
Yu, Jiacheng1,2,3; Zhang, Tongwei1,2; Xu, Huangtao1,2,3; Dong, Xiaoli4; Cai, Yao1,2; Pan, Yongxin1,2,3; Cao, Changqian1,2,3
2019-12-01
Source PublicationRSC ADVANCES
Volume9Issue:67Pages:39381-39393
AbstractThermostable nanoparticles have numerous applications in catalysis and in the oil/gas industry. However, synthesizing these nanoparticles requires expensive polymers. Here, a novel thermostable ferritin named PcFn, originally from the hyperthermophilic archaeon Pyrococcus yayanosii CH1, was overexpressed in Escherichia coli, purified and characterized, which could successfully direct the synthesis of thermostable magnetoferritins (M-PcFn) with monodispersed iron oxide nanoparticles in one step. Transmission electron microscopy and magnetic measurements show that the cores of the M-PcFn have an average diameter of 4.7 nm, are well-crystalline and superparamagnetic. Both the PcFn and M-PcFn can resist temperatures up to 110 degrees C, which is significantly higher than for human H-chain ferritin (HFn) and M-HFn, and comparable to temperatures previously reported for Pyrococcus furiosus ferritin (PfFn) and M-PfFn. After heating at 110 degrees C for 30 minutes, PcFn and M-PcFn maintained their secondary structures and PcFn retained 87.4% of its iron uptake activity. This remarkable thermostability of PcFn and M-PcFn suggests potential applications in elevated temperature environments.
DOI10.1039/c9ra07397c
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences
WOS KeywordAMINO-ACID PROPERTIES ; THERMAL-STABILITY ; PROTEIN CAGE ; TEMPERATURE ; MAGNETOFERRITIN ; STABILIZATION ; NANOCLUSTERS ; UNIFORM ; BRINE ; SIZE
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[41574062] ; National Natural Science Foundation of China (NSFC)[41774076] ; National Natural Science Foundation of China (NSFC)[41621004] ; Key Program of Chinese Academy of Sciences[QYZDJ-SSW-DQC024]
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000501820700048
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iggcas.ac.cn/handle/132A11/95840
Collection地球与行星物理院重点实验室
Corresponding AuthorCao, Changqian
Affiliation1.Chinese Acad Sci, Phys Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Biogeomagnetism Grp,Paleomagnetism & Geochronol L, Beijing 100029, Peoples R China
2.Chinese Acad Sci, Innovat Acad Earth Sci, Beijing 100029, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
4.Univ Calgary, Dept Geosci, Calgary, AB T2N 1N4, Canada
Recommended Citation
GB/T 7714
Yu, Jiacheng,Zhang, Tongwei,Xu, Huangtao,et al. Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1[J]. RSC ADVANCES,2019,9(67):39381-39393.
APA Yu, Jiacheng.,Zhang, Tongwei.,Xu, Huangtao.,Dong, Xiaoli.,Cai, Yao.,...&Cao, Changqian.(2019).Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1.RSC ADVANCES,9(67),39381-39393.
MLA Yu, Jiacheng,et al."Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1".RSC ADVANCES 9.67(2019):39381-39393.
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