IGGCAS OpenIR  > 地球与行星物理院重点实验室
Unveiling of active diazotrophs in a flooded rice soil by combination of NanoSIMS and(15)N(2)-DNA-stable isotope probing
Wang, Xiaojie1; Bei, Qicheng1; Yang, Wei2; Zhang, Heng3,4; Hao, Jialong2; Qian, Li5; Feng, Yucheng6; Xie, Zubin1
2020-08-10
Source PublicationBIOLOGY AND FERTILITY OF SOILS
ISSN0178-2762
Pages11
AbstractThe biological nitrogen fixation in planted and nonplanted paddy soils was quantified using a chamber-based(15)N(2)-labeling technique, and the active diazotrophs of soil were assessed by(15)N(2)-DNA-stable isotope probing (SIP). In addition, the nanometer scale secondary ion mass spectrometry (NanoSIMS) was applied to analysis the(15)N-enrichment of soil DNA in SIP fractions.N-15(2)-labeling experiment showed that BNF was 11.33 +/- 1.90 kg N ha(-1)in the rice-planted soil and 3.55 +/- 1.18 kg N ha(-1)in the nonplanted soil after 28-day labeling. The biologically fixed(15)N was mainly (> 95%) recovered in the surface layer (0-0.5 cm) in the rice-planted soil. High throughput sequencing ofnifHgenes extracted from surface soil showed that the presence of rice affected the community composition of diazotrophs. The relative abundance ofNostocalesandStigonemataleswas significantly higher in rice-planted soil than in nonplanted soil (P< 0.05). After CsCl gradient ultracentrifugation, NanoSIMS images clearly showed that(15)N was incorporated into soil DNA in the(15)N(2)-labeling SIP gradient fractions. Analyses ofnifHgenes in(15)N-enriched SIP gradient fractions suggested thatNostocalesandStigonemataleswere the major contributors to BNF in the rice-soil system. Taken together, these results have highlighted the contributions of cyanobacteria to the BNF in paddy fields and improved our understanding of the close relationship between rice plants and cyanobacteria.
KeywordN-15(2)-labeling DNA-SIP NanoSIMS Cyanobacteria Rice soil
DOI10.1007/s00374-020-01497-2
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province
WOS KeywordBIOLOGICAL NITROGEN-FIXATION ; NIFH GENE POOLS ; MASS-SPECTROMETRY ; CYANOBACTERIAL DIVERSITY ; LIQUID-CHROMATOGRAPHY ; NUCLEIC-ACIDS ; GROWTH ; COMMUNITIES ; RHIZOSPHERE ; ROOTS
Language英语
Funding ProjectNatural Science Foundation of China[31870500] ; Natural Science Foundation of China[41501273] ; Special Project on the Basis of National Science and Technology of China[2015FY110700] ; Knowledge Innovation Program of Chinese Academy of Sciences[KZCX2-EW-409] ; Technology Supporting Project of Jiangsu Province[BE2013451]
Funding OrganizationNatural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Special Project on the Basis of National Science and Technology of China ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Knowledge Innovation Program of Chinese Academy of Sciences ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province ; Technology Supporting Project of Jiangsu Province
WOS Research AreaAgriculture
WOS SubjectSoil Science
WOS IDWOS:000558108400001
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.iggcas.ac.cn/handle/132A11/97630
Collection地球与行星物理院重点实验室
Corresponding AuthorBei, Qicheng; Yang, Wei; Xie, Zubin
Affiliation1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China
2.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China
3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China
4.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai 201602, Peoples R China
5.Nanjing Agr Univ, Inst Resources Ecosyst & Environm Agr, Nanjing 210095, Peoples R China
6.Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA
Corresponding Author AffilicationInstitute of Geology and Geophysics, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiaojie,Bei, Qicheng,Yang, Wei,et al. Unveiling of active diazotrophs in a flooded rice soil by combination of NanoSIMS and(15)N(2)-DNA-stable isotope probing[J]. BIOLOGY AND FERTILITY OF SOILS,2020:11.
APA Wang, Xiaojie.,Bei, Qicheng.,Yang, Wei.,Zhang, Heng.,Hao, Jialong.,...&Xie, Zubin.(2020).Unveiling of active diazotrophs in a flooded rice soil by combination of NanoSIMS and(15)N(2)-DNA-stable isotope probing.BIOLOGY AND FERTILITY OF SOILS,11.
MLA Wang, Xiaojie,et al."Unveiling of active diazotrophs in a flooded rice soil by combination of NanoSIMS and(15)N(2)-DNA-stable isotope probing".BIOLOGY AND FERTILITY OF SOILS (2020):11.
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